Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: Sequence of primers for the internal reference gene and circular RNAs (circRNAs)

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques: Sequencing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: Differential expression of circular RNAs (circRNAs). (A) The scatter plot is a visualization method used for assessing the variation in circRNA expression. The values corresponding to the X ‐axis and Y ‐axis in the scatter plot are the normalized signal values of the samples (log2 scaled). The green lines indicate fold changes. The circRNAs above the top green line and below the bottom green line indicate more than 2.0‐fold changes between the two groups. (B) Volcano plots were constructed using fold‐change values and P ‐values. The vertical lines correspond to 2.0‐fold over‐ and under‐expression between two groups, and the horizontal line represents a threshold P ‐value. The red points in the plot represent significantly differentially expressed circRNAs ( P < .05)

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques: Quantitative Proteomics, Expressing, Construct

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: Gene ontology (GO) and subcellular locations of differentially expressed circular RNAs (circRNAs). GO analysis enrichment of (A) biological processes, (B) cellular components (C) molecular functions; (D) Pathways corresponding to under‐expressed circRNA transcripts. The bar graph shows the top enrichment score value of the significantly enriched pathway

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques:

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: Differential expression of circular RNAs (circRNAs) in plasma between two groups

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques: Quantitative Proteomics, Clinical Proteomics, Control

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: The expression levels of circular RNAs (circRNAs) in cases with congenital heart diseases (CHD) and healthy controls. Expression histogram (A) plasma hsa_circRNA_004183, (B) plasma hsa_circRNA_079265, and (C) plasma hsa_circRNA_105039. * P < .05

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques: Expressing, Clinical Proteomics

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: The sensitivity, specificity, and area under curve (AUC) of receiver operating characteristic (ROC) curves of three circular RNAs (circRNAs) individually and combined

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques:

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating plasma circular RNAs as novel diagnostic biomarkers for congenital heart disease in children

doi: 10.1002/jcla.22998

Figure Lengend Snippet: Circular RNAs (circRNAs)‐miRNA‐mRNA interaction network. The network consists of 9 nodes (genes). In the circRNA‐miRNA‐mRNA network, the inverted triangles represent circRNAs, circles represent mRNAs, and rhombuses represent miRNAs. An interaction between two factors is represented by one edge

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2.0 (8x15K, Arraystar).

Techniques:

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: Primer sequences used for qRT-PCR analysis of circRNA and mRNA levels.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques:

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: CircRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of circRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the scatterplot, the values on the X - and Y -axes are the log2 scaled signals of samples. Fold change is represented by the green lines. The circRNAs outside the range formed by the upper and lower green lines are those with the fold change ≥ 1.5 between the compared groups. In the volcano plot, the vertical green lines represent a 1.5-fold change although the horizontal green line corresponds to a P -value of 0.05. The red points in the volcano plot represent the significantly altered circRNAs with P -value <0.05. (C) Different subgroups of significantly altered circRNAs according to their effects and position. (D) Heat maps of circRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates a single circRNA, each column indicates a single sample.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Expressing, Microarray

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: mRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of mRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the plot, red and green points represent significant altered mRNAs (fold change ≥ 1.5, P -value < 0.05), respectively. (C) Heat maps of mRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates single circRNA; each column indicates single sample.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Expressing, Microarray

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: Selected circRNA and mRNA qRT-PCR verification between the IRI and sham groups (A) and between the IPC and IRI groups (B) . Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham, and ** P <0.01 compared with the sham group; ## P <0.01 compared with the IRI group.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Quantitative RT-PCR

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: Identification of circRNAs related to hepatic protection by IPC and qRT-PCR validation. (A) The Venn diagram shows the circRNAs with an opposite direction of the alterations between the two comparison groups (sham vs. IRI and IRI vs. IPC). (B) qRT-PCR detected expression levels of circRNA_017753 in mouse liver among the three groups. Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham group; # P <0.05 compared with the IRI group.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Quantitative RT-PCR, Expressing

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: The identified circRNAs and its predicted miRNA response elements (MREs).

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques:

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: (A) Prediction of MiRNA and the circRNA–miRNA–mRNA pathway. Detailed structure of circRNA/miRNA interactions. The MRE sequence, miRNA seed type, precise base positions and target miRNA nucleotides are presented as annotation in 2-D structure column. Thirty nucleotides downstream and upstream the seed sequence are presented in “local AU.” Red bars present A/U, and black bars present G/C. The most likely relative MRE position are presented in position column. (B) Overlap of the predicted mRNA target related to circRNA_017753 and significantly upregulated mRNAs caused by IPC intervention.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Sequencing

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet:

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques:

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet:

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: